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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Monday, 14 February 2011

D29, Bxz2 and TM4 mycobacterium phages again





At present there are not phages for Mycobacterium ulcerans except that Bxz2 and D29( L5 and TM4).



D29



Organism Information

Organism Name Mycobacteria phage D29
Taxon Object ID 638276458
NCBI Taxon ID 28369
External Links NCBI/RefSeq:NC_001900; PUBMED:9636706
Genome type isolate
Lineage Viruses; dsDNA viruses, no RNA stage; unclassified; Caudovirales; Siphoviridae; L5-like viruses; phage
Sequencing Status Finished
IMG Release IMG/W 2.0
Comment


Number % of Total
DNA, total number of bases 49136 100.00%
DNA coding number of bases 44927 91.43%
DNA G+C number of bases 31216 63.53% 1



DNA scaffolds 1 100.00%



Genes total number 84 100.00%
Protein coding genes 79 94.05%
RNA genes 5 5.95%
tRNA genes 5 5.95%
Protein coding genes with function prediction 42 50.00%
Protein coding genes without function prediction 37 44.05%
Genes w/o function with similarity 33 39.29%
Genes w/o function w/o similarity 4 4.76%
Protein coding genes connected to SwissProt Protein Product 15 17.86%
not connected to SwissProt Protein Product 64 76.19%
Protein coding genes connected to SEED 79 94.05%
Protein coding genes with enzymes 3 3.57%
w/o enzymes but with candidate KO based enzymes 6 7.14%
Protein coding genes connected to KEGG pathways3 3 3.57%
not connected to KEGG pathways 76 90.48%
Protein coding genes connected to KEGG Orthology (KO) 4 4.76%
not connected to KEGG Orthology (KO) 75 89.29%
Protein coding genes connected to MetaCyc pathways 1 1.19%
not connected to MetaCyc pathways 78 92.86%
Protein coding genes with COGs3 14 16.67%
with Pfam3 24 28.57%
with TIGRfam3 3 3.57%
with InterPro 17 20.24%
with IMG Terms 2 2.38%
Protein coding genes in ortholog clusters 75 89.29%
Protein coding genes coding signal peptides 14 16.67%
Protein coding genes coding transmembrane proteins 6 7.14%
COG clusters 13 0.27%
Pfam clusters 24 0.20%
TIGRfam clusters 3 0.07%
Paralogous groups 0 100.00%
Orthologous groups 75 0.02%

Notes:
1 - GC percentage shown as count of G's and C's divided by a total number of G's, C's, A's, and T's.
This is not necessarily synonymous with the total number of bases.
2 - Pseudogenes may also be counted as protein coding or RNA genes,
so is not additive under total gene count.
3 - Graphical view available.

Bxz2



Organism Information

Organism Name Mycobacterium phage Bxz2
Taxon Object ID 638276476
NCBI Taxon ID 205870
External Links NCBI/RefSeq:NC_004682; PUBMED:12705866
Genome type isolate
Lineage Viruses; dsDNA viruses, no RNA stage; unclassified; Caudovirales; Siphoviridae; unclassified; phage
Sequencing Status Finished
IMG Release IMG/W 2.0
Comment


Number % of Total
DNA, total number of bases 50913 100.00%
DNA coding number of bases 46433 91.20%
DNA G+C number of bases 32677 64.18% 1



DNA scaffolds 1 100.00%



Genes total number 89 100.00%
Protein coding genes 86 96.63%
RNA genes 3 3.37%
tRNA genes 3 3.37%
Protein coding genes with function prediction 86 96.63%
not connected to SwissProt Protein Product 86 96.63%
Protein coding genes connected to SEED 86 96.63%
Protein coding genes with enzymes 3 3.37%
w/o enzymes but with candidate KO based enzymes 5 5.62%
Protein coding genes connected to KEGG pathways3 3 3.37%
not connected to KEGG pathways 83 93.26%
Protein coding genes connected to KEGG Orthology (KO) 3 3.37%
not connected to KEGG Orthology (KO) 83 93.26%
Protein coding genes connected to MetaCyc pathways 1 1.12%
not connected to MetaCyc pathways 85 95.51%
Protein coding genes with COGs3 13 14.61%
with Pfam3 23 25.84%
with TIGRfam3 2 2.25%
with InterPro 16 17.98%
with IMG Terms 1 1.12%
Protein coding genes in ortholog clusters 74 83.15%
Protein coding genes coding signal peptides 16 17.98%
Protein coding genes coding transmembrane proteins 7 7.87%
COG clusters 12 0.25%
Pfam clusters 25 0.21%
TIGRfam clusters 2 0.05%
Paralogous groups 0 100.00%
Orthologous groups 74 0.02%

Notes:
1 - GC percentage shown as count of G's and C's divided by a total number of G's, C's, A's, and T's.
This is not necessarily synonymous with the total number of bases.
2 - Pseudogenes may also be counted as protein coding or RNA genes,
so is not additive under total gene count.
3 - Graphical view available.







TM4


Mycobacteriophage Database

Organism Information

Organism Name Mycobacterium phage TM4
Taxon Object ID 638276485
NCBI Taxon ID 88870
External Links NCBI/RefSeq:NC_003387; PUBMED:10645443
Genome type isolate
Lineage Viruses; dsDNA viruses, no RNA stage; unclassified; Caudovirales; Siphoviridae; unclassified; phage
Sequencing Status Finished
IMG Release IMG/W 2.0
Comment


Number % of Total
DNA, total number of bases 52797 100.00%
DNA coding number of bases 48528 91.91%
DNA G+C number of bases 35962 68.11% 1



DNA scaffolds 1 100.00%



Genes total number 89 100.00%
Protein coding genes 89 100.00%
Protein coding genes with function prediction 7 7.87%
Protein coding genes without function prediction 82 92.14%
Genes w/o function with similarity 77 86.52%
Genes w/o function w/o similarity 5 5.62%
not connected to SwissProt Protein Product 89 100.00%
Protein coding genes connected to SEED 89 100.00%
w/o enzymes but with candidate KO based enzymes 2 2.25%
not connected to KEGG pathways 89 100.00%
Protein coding genes connected to KEGG Orthology (KO) 1 1.12%
not connected to KEGG Orthology (KO) 88 98.88%
not connected to MetaCyc pathways 89 100.00%
Protein coding genes with COGs3 5 5.62%
with Pfam3 14 15.73%
with TIGRfam3 3 3.37%
with InterPro 10 11.24%
with IMG Terms 2 2.25%
with IMG Parts List 1 1.12%
Protein coding genes in ortholog clusters 75 84.27%
Protein coding genes coding signal peptides 16 17.98%
Protein coding genes coding transmembrane proteins 10 11.24%
COG clusters 5 0.10%
Pfam clusters 14 0.12%
TIGRfam clusters 3 0.07%
Paralogous groups 0 100.00%
Orthologous groups 75 0.02%

Notes:
1 - GC percentage shown as count of G's and C's divided by a total number of G's, C's, A's, and T's.
This is not necessarily synonymous with the total number of bases.
2 - Pseudogenes may also be counted as protein coding or RNA genes,
so is not additive under total gene count.
3 - Graphical view available.