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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Sunday, 27 February 2011

Mycobacterium ulcerans Agy99 from Integrated Microbial Genome

Organism Information



0rganism Name Mycobacterium ulcerans Agy99
Taxon Object ID 642555140
NCBI Taxon ID 362242
RefSeq Project ID 16230
GenBank Project ID 16230
GOLD ID in IMG Database Gc00469
External Links NCBI/RefSeq:NC_005916; NCBI/RefSeq:NC_008611; PUBMED:14736915; PUBMED:17210928
Genome type isolate
Lineage Bacteria; Actinobacteria; Actinobacteria; Actinomycetales; Mycobacteriaceae; Mycobacterium; ulcerans
Sequencing Status Finished
IMG Release IMG/W 2.7
Comment
Project Information
Geographic Location ulcerative lesion on the right elbow of a female patient from the Ga district of Ghana in 1999
GOLD ID Gc00469
Isolation Country Ghana
Isolation Year July, 1999
NCBI Project ID 16230
Publication Journal Genome Research (17, 192-200)
Sequencing Center Institut Pasteur
Project Sequencing Method Sanger
Metadata
Assembly Method Phrap, GAP4
Biotic Relationships Free living
Cell Shape Rod-shaped
Gram Staining Gram+
Host Name Homo sapiens
Host Gender Female
Host Health Patient
Isolation ulcerative lesion on the right elbow of a female patient from the Ga district of Ghana in 1999
Library Method 2-3Kb, 3-5Kb, 5-10Kb
Motility Nonmotile
Oxygen Requirement Aerobe
Sporulation Nonsporulating
Temperature Range Mesophile
Temperature Optimum 32
Cell Arrangement Singles
Diseases Buruli ulcer
Energy Source Chemoorganotroph
Habitat Host, Fresh water
Phenotype Pathogen, Antibiotic resistant
Relevance Medical, Human Pathogen
Body Site Skin
Phenotypes/Metabolism from Pathway Assertion
Metabolism Auxotroph (L-lysine auxotroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-alanine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-aspartate prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-glutamate prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Auxotroph (L-phenylalanine auxotroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Auxotroph (L-tyrosine auxotroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Auxotroph (L-tryptophan auxotroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Auxotroph (L-histidine auxotroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Auxotroph (Glycine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-arginine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-asparagine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-cysteine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-glutamine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-isoleucine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Auxotroph (L-leucine auxotroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-proline prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-serine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-threonine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (L-valine prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism (Non-selenocysteine synthesizer) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (Biotin prototroph) (IMG_PIPELINE; 2011-01-04)
Metabolism Prototrophic (Coenzyme A prototroph) (IMG_PIPELINE; 2011-01-04)




Number % of Total
DNA, total number of bases 5805761 100.00%
DNA coding number of bases 4236262 72.97%
DNA G+C number of bases 3796479 65.39% 1



DNA scaffolds 2 100.00%
Plasmid Count 1



Genes total number 4306 100.00%
Protein coding genes 4241 98.49%
RNA genes 65 1.51%
rRNA genes 3 0.07%
5S rRNA 1 0.02%
16S rRNA 1 0.02%
23S rRNA 1 0.02%
tRNA genes 45 1.05%
Other RNA genes 17 0.39%
Protein coding genes with function prediction 2792 64.84%
Protein coding genes without function prediction 1449 33.65%
Genes w/o function with similarity 1443 33.51%
Genes w/o function w/o similarity 6 0.14%
Protein coding genes connected to SwissProt Protein Product 331 7.69%
not connected to SwissProt Protein Product 3910 90.80%
Protein coding genes connected to SEED 353 8.20%
not connected to SEED 3888 90.29%
Protein coding genes with enzymes 983 22.83%
w/o enzymes but with candidate KO based enzymes 276 6.41%
Protein coding genes connected to Transporter Classification 305 7.08%
Protein coding genes connected to KEGG pathways3 1055 24.50%
not connected to KEGG pathways 3186 73.99%
Protein coding genes connected to KEGG Orthology (KO) 1790 41.57%
not connected to KEGG Orthology (KO) 2451 56.92%
Protein coding genes connected to MetaCyc pathways 761 17.67%
not connected to MetaCyc pathways 3480 80.82%
Protein coding genes with COGs3 2853 66.26%
with Pfam3 3420 79.42%
with TIGRfam3 1071 24.87%
with InterPro 1798 41.76%
with IMG Terms 777 18.04%
with IMG Pathways 313 7.27%
with IMG Parts List 265 6.15%
Protein coding genes in ortholog clusters 3982 92.48%
in paralog clusters 959 22.27%
in Chromosomal Cassette 3976 92.34%
Number of Chromosomal Cassettes 673 -
Fused Protein coding genes 352 8.17%
as fusion components 2108 48.95%
Protein coding genes coding signal peptides 1141 26.50%
Protein coding genes coding transmembrane proteins 839 19.48%
COG clusters 1379 28.30%
Pfam clusters 1542 12.94%
TIGRfam clusters 879 21.85%
Paralogous groups 235 100.00%
Orthologous groups 3905 1.02%


Notes:
1 - GC percentage shown as count of G's and C's divided by a total number of G's, C's, A's, and T's.
This is not necessarily synonymous with the total number of bases.
2 - Pseudogenes may also be counted as protein coding or RNA genes,
so is not additive under total gene count.
3 - Graphical view available.