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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Monday, 17 May 2010

Receptors....?


From :
"A peptidoglycan hydrolase motif within the mycobacteriophage TM4 tape measure protein promotes efficient infection of stationary phase cells "







See table from the scientific work above-mentioned:




These are the alignments of these proteins by Jalview (open source) and Geneious.








"Within these phage genomes the tape measure protein (tmp) gene can be readily identified due to the well-established relationship between the length of the gene and the length of the phage tail – because these phages typically have long tails, the tmp gene is usually the largest gene in the genome."

I have compared TM4gp17 sequence with the genomes of D29, L5 and Bxz2 mycobacterium phages and with some proteins like to TM4gp17 for checking if these proteins contain a region that is also the receptor used in the adsorption on the bacterial cells. These proteins are:














This is the alignment of these genes by MAUVE (open source).


By mEMBOSS I have found the composition and the total charge for each protein:



Charge:







See Mpr protein.

By mEMBOSS software all these proteins have transmembrane regions
:




These are the alignments of these proteins by Jalview, Mauve and Geneious.













Similarity and Identity









I have used TmrPres2d software (open source) incorrectly only for drawing the transmembrane regions in the protein chain:





In this way it is possible to observe the charge distribution on the protein chain and also to realize if these areas may be the regions of the phage receptor:





D29p24









This is the alignment of these small portions by Jalview (open source).