The problem is to identify the phage receptor.
If Mpr protein of M.smegmatis arrests the growth of D29 and L5 mycobacterium phages then this protein may be involved with the phage receptors and also it must a transmembrane protein.
Mpr protein (multicopy phage resistance) as the capacity to react with Phage proteins and to arrest the Phage cycle.
Elevation of Mpr is thought to cause resistance to D29 infection by inhibiting transport of phage DNA across the cell wall.
Over expression of this gene also confers resistance to L5 and partial resistance to TM4 but does not effect infection by Bxb1 or I3 (Barsom and Hatfull 1996).
Over expression of this gene also confers resistance to L5 and partial resistance to TM4 but does not effect infection by Bxb1 or I3 (Barsom and Hatfull 1996).
1-copy the protein sequence in Tmap program ( predict and plot transmembrane segments in protein sequences, Jemboss software)
2-safe the result
3- copy the protein sequence in MPEx ( Membrane Protein Explorer,open source)
4-safe the result
5- copy the protein sequence in TMRPres2D software
6-safe the image
