Phage Therapy (Biocontrol*): Light and Shade Visit new site : Sellanophagetherapy.blogspot.com: M.smegmatis Mpr protein alignment with M.ulcerans and M.marinum proteins

"In silico"

From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.

Thursday, 27 May 2010

M.smegmatis Mpr protein alignment with M.ulcerans and M.marinum proteins

1-By BugView software (open source) I have compared Mpr protein from M. smegmatis str. MC2 155 with genomes of M.ulcerans Agy99 and M.marinum M.

2-By Geneious software ( commercial software) I have studied the proteins that have a correlation with Mpr protein:

MUL 3786- 4227021:4227812 proline rich membrane protein

MMAR_3854 - 4791940: 4792731 proline rich membrane protein

Alignments

By Gepard software (open source) : Dot plot

E	Enterococcus faecium	Third most common cause of HCA BSI. Increasing resistance to vancomycin.
S	Staphylococcus aureus (MRSA)	Emerging resistance to current drugs and significant drug toxicities. Lack of oral agents for step-down therapy
K	Klebsiella Escherichia coli K pneumoniae	ESBL-producing organisms increasing in frequency and severity; associated with increasing mortality. K pneumoniae carbapenemases causing severe infections in LTCF. Few active agents; nothing in development
A	Acinetobacter baumannii	Increasing worldwide, recent surge reported in hospitals. Very high mortality. Carbapenem-resistant.
P	Pseudomonas aeruginosa	Increasing P. aeruginosa infections in US and worldwide. Resistant to carbapenems, quinolones, aminoglycosides
E	Enterobacter species	MDR HCA infections increasing; resistance via ESBLs, carbapenemases, and cephalosporinases

E	Enterococcus faecium
S	Staphylococcus aureus (MRSA)	+
K	Klebsiella Escherichia coli K pneumoniae
A	Acinetobacter baumannii	+
P	Pseudomonas aeruginosa	++
E	Enterobacter species

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messaggio

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"In silico"

Phage Therapy is influenced by:

Thursday, 27 May 2010

M.smegmatis Mpr protein alignment with M.ulcerans and M.marinum proteins