Thursday, 24 December 2009

Which is the Phage receptor? Third part.

Third comparison:BXZ2 phage genome

The Third comparison is with BXZ2 phage genome:

1- this is the result of Local (Smith-Waterman) comparison between the whole BXZ2 sequence and Proteins of Mycobacterium ulcerans Agy99:



By BugView:
4208 comparisons for user's query sequence and Mycobacterium ulcerans Agy99.
Finished in 4376 seconds.

Highest score = 1516 for cds_3629 (118619508)
Second score = 1108 for cds_967 (118616873)
Third score = 979 for cds_3641 (118619519)



2
-I have compared ,one by one, the indicated M.ulcerans cds with the
BXZ2 phage genome:


1°cds_3629


cds_3629 is (MUL_4367):

PE-PGRS family protein (Mycobacterium ulcerans Agy99)
Other Aliases: MUL_4367
Annotation: NC_008611.1 (4845511..4848744, complement)
GeneID: 4550406


By BugView:
89 comparisons for cds_3629 (118619508)
Finished in 1 seconds.

Highest score = 406 for cds_3 (29566025)
Second score = 247 for cds_4 (29566026)
Third score = 110 for cds_26 (29566045)

cds_3

Bxz2_gp4
gp4 [Mycobacterium phage Bxz2]
Other Aliases: Bxz2_gp4, Bxz2p04
Annotation: NC_004682.1 (1692..2726)
GeneID: 1259216


Accession.......... GeneID

NP_817595.11259216





I have compared , the indicated cds 3 with the M.ulcerans genome:

By BugView:

4208 comparisons for cds_3 (29566025)
Finished in 46 seconds.

Highest score = 414 for cds_3076 (118618968)
Second score = 413 for cds_620 (118616526)

Third score = 406 for cds_3629 (118619508)





2°cds_967

By BugView:
89 comparisons for cds_967 (118616873)
Finished in 0 seconds.

Highest score = 281 for cds_3 (29566025)
Second score = 212 for cds_4 (29566026)
Third score = 111 for cds_26 (29566045)







3°cds_3641


By BugView:

89 comparisons for cds_3641 (118619519)
Finished in 1 seconds.

Highest score = 383 for cds_3 (29566025)
Second score = 208 for cds_4 (29566026)
Third score = 118 for cds_26 (29566045)






In the cluster is present the gp5 from cds 4. But the gp4 from cds 3 is correlated to the gp5.

Accession................. GeneID

NP_817596.11259217