Thursday, 24 December 2009

Which is the Phage receptor? Fourth part.

Fourth comparison:TM4 phage genome

The Fourth comparison is with TM4 phage genome:

1- this is the result of Local (Smith-Waterman) comparison between the whole TM4 sequence and Proteins of Mycobacterium ulcerans Agy99:


By BugView
:
4208 comparisons for user's query sequence and
Mycobacterium ulcerans Agy99
Finished in 4629 seconds.

Highest score = 1695 for cds_3629 (118619508)
Second score = 1217 for cds_2549 (118618448)
Third score = 1094 for cds_3641 (118619519)



2-I have compared ,one by one, the indicated M.ulcerans cds with the TM4 phage genome:


1°cds_3629


cds_3629 is (MUL_4367):

PE-PGRS family protein (Mycobacterium ulcerans Agy99)
Other Aliases: MUL_4367
Annotation: NC_008611.1 (4845511..4848744, complement)
GeneID: 4550406



By BugView:
89 comparisons for cds_3629 (118619508)
Finished in 1 seconds.

Highest score = 266 for cds_24 (18496911)
Second score = 150 for cds_22 (18496909)
Third score = 128 for cds_8 (18496895)



cds_24


TM4_gp25
similar to L5 gp32 [Mycobacterium phage TM4]
Other Aliases: TM4_gp25, TM4_25
Other Designations: hypothetical protein
Annotation: NC_003387.1 (21422..22426)
GeneID: 932261



Accession...... GeneID

NP_569761.1932261




I have compared , the indicated cds 24 with the M.ulcerans genome:

By BugView
:
4208 comparisons for cds_24 (18496911)
Finished in 48 seconds.

Highest score = 299 for cds_331 (118616247)
Second score = 279 for cds_3309 (118619191)
Third score = 277 for cds_620 (118616526)






2°cds_2549

By BugView:
89 comparisons for cds_2549 (118618448)
Finished in 1 seconds.

Highest score = 244 for cds_24 (18496911)
Second score = 171 for cds_22 (18496909)
Third score = 137 for cds_28 (18496915)







3°cds_3641



By BugView:
89 comparisons for cds_3641 (118619519)
Finished in 1 seconds.

Highest score = 252 for cds_24 (18496911)
Second score = 163 for cds_22 (18496909)
Third score = 140 for cds_8 (18496895)