Thursday, 24 December 2009

Which is the Phage receptor? Second part.




Second comparison
:L5 phage genome


The second comparison is with L5 phage genome:

1- this is the result of Local (Smith-Waterman) comparison between the whole L5 sequence and Proteins of Mycobacterium ulcerans Agy99:


By BugView:
4208 comparisons for user's query sequence and Mycobacterium ulcerans Agy99.Finished in 4126 seconds.

Highest score = 1559 for
cds_3629 (118619508)
Second score = 1108 for
cds_2549 (118618448)
Third score = 993 for
cds_3641 (118619519)


2-I have compared ,one by one, the indicated M.ulcerans cds with the L5 genome:


1°cds_3629
cds_3629 is (MUL_4367):

PE-PGRS family protein (Mycobacterium ulcerans Agy99)
Other Aliases: MUL_4367
Annotation: NC_008611.1 (4845511..4848744, complement)
GeneID: 4550406

By BugView:
88 comparisons for cds_3629 (118619508)
Finished in 1 seconds.

Highest score = 244 for cds_31 (9625462)
Second score = 126 for cds_27 (9625458)
Third score = 104 for cds_9 (9625440



cds_31

L5p32
gene 32 [Mycobacterium phage L5]
Other Aliases: L5p32
Other Designations: predicted 21.3kd protein
Annotation: NC_001335.1 (23536..24198)
GeneID: 2942918

Accession............... GeneID.

NP_039696.12942918




I have compared , the indicated cds 31 with the M.ulcerans genome:



By
BugView
:
4208 comparisons for cds_31 (9625462).
Finished in 37 seconds.

Highest score = 264 for cds_3679 (118619555)
Second score = 261 for cds_3560 (118619440)
Third score = 261 for cds_2695 (118618593)





2°cds_2549




By BugView:
88 comparisons for cds_2549 (118618448)
Finished in 0 seconds.
Highest score = 234 for cds_31 (9625462)
Second score = 119 for cds_30 (9625461)
Third score = 107 for cds_27 (9625458)




3°cds_3641

By BugView:
88 comparisons for cds_3641 (118619519)
Finished in 1 seconds.

Highest score = 234 for cds_31 (9625462)
Second score = 111 for cds_11 (9625442)
Third score = 109 for cds_27 (9625458)