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"In silico"


 From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
 Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Sunday, 2 January 2011

To read up again and again





From this web site:


Classic Readings

The following are PDF files of some classic phage papers.

Adams, M. H., 1955. Abortive Infection with Phage T2 at Low Temperatures. Virology 1: 336-346.


Adams, M. H., 1959. Ch. VIII. Antigenic Properties, pp. 97-119 in Bacteriophages. Interscience Publishers, Inc., London.


Craigie, J. and Brandon, K. F., 1936. The Laboratory Identification of V Form of B. Typhosus, pp. in 4th Annual Christmas Meeting of the Laboratory Section. Canadian Public Health Association, Toronto.


Desranleau, J.-M., 1949. Progress in the Treatment of Typhoid Fever with Vi Phages, pp. 473-478 in Candian Journal of Public Health, Novia Scotia, Halifax, Cananda.


Dubos, R., Straus, J. H. and Pierce, C., 1943. The multiplication of bacteriophage in vivo and its protective effect against an experimental infection with Shigella dysenteriae. J Exp Med 20: 161-168.


Hershey, A. D. and Chase, M., 1952. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. Gen. Physiol. 36: 39-56.


Hotchin, J. E., 1951. The influence of acridines on the interaction of Staphylococcus aureus and Staphylococcus K phage. JGM 5: 609-618.


Hotchin, J. E. , Dawson, I. M., and Elford, W. J., 1951. Use of empty bacterial membranes in the study of the adsoprtion of Staphylococcus K phage upon its host. British Journal of Experimental Pathology. pp. 177-182.


Knouf, E. G., Ward, W. E., Reichle, P. A., Bower, A. G. and Hamilton, P. M., 1946. Treatment of typhoid fever with type-specific bacteriophage. J Am Med Assn 132: 134-136.


Morton, H. E. and Engely, F. B., 1945. Dysentery Bacteriophage: Review of the Literature on its Prophylactic and Therapeutic Uses in Man and in Experimental Infections in Animals. J Am Med Assoc 127: 584-591.



Morton, H. E. and Perez-Otero, J. E., 1944. The Increase of Bacteriophage in Vivo During Experimantal Infections with Shigella paradysenteriae, Flexner, in mice. J Bacteriol 47: 475-476.


Rees, P. J., and Fry, B. A., 1981. The morphology of Staphylococccal bacteriophage K and DNA metabolism in infected Staphylococcus aureus. JGV 53: 293-307.


Rees, P. J., and Fry, B. A., 1981. The replication of bacteriophage K DNA in Staphylococcus aureus. JGV 55: 41-51.


Rees, P. J., and Fry, B. A., 1983. Structure and properties of the rapidly sedimenting replicating complex of Staphylococcal phage K DNA. JGV 64: 191-198.


Schade, A. L. and Caroline, L., 1943. The preparation of a polyvalent dysentery bacteriophage in a dry and stable form. I. Preliminary Investigations and general procedures. J Bacteriol 46: 463-473.




Schade, A. L. and Caroline, L., 1944. The preparation of a polyvalent dysentery bacteriophage in a dry and stable form. II. Factors affecting the stabilization of dysentery bacteriophage during lyophilization. J Bacteriol 48: 179-190.


Schade, A. L. and Caroline, L., 1944. The preparation of a polyvalent dysentery bacteriophagein a dry and stable form. III. Stability of the dried bacteriophage towards heat humidity age and acididty. J Bacteriol: 243-251.



Simon, L. D., 1969. The Infection of Escherichia coli by T2 and T4 Bacteriophages as Seen in the Electron Microscope. III. Membrane-Associated Intracellular Bacteriophages. Virology 38: 285-296.


Ward, W. E., 1942. Protective Action Of VI Bacteriophage In Eberthella Typhi Infections In Mice. Journal of Infectious Disease: 172-176.

Posted by Rodolfo Berretti at 17:02
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