Mycobacterium marinum and Mycobacterium ulcerans:pater et filius

To find "genuine" lytic mycobacteriophages is not easy and it seems to me that it is also rare .

D29 is a lytic phage but I can not consider this phage a "genuine " lytic phage by genome analysis.

Mycobacteriophages ( D29,L5,Bxz2 and L5) have a broad Host range and this feature is not favourable when they are used in Phage therapy.

I am of the opinion that Mycobacterium marinum may be the valid alternative to Mycobacterium smegmatis for growing and isolating phages with a potential action on Mycobacterium ulcerans but until now there is little information about growing phages on Mycobacterium marinum.

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M.marinum,M.ulcerans and M.smegmatis:In silico 2D-SDS-PAGE

Mycobacterium marinum M

Replicon	Total Genes	Protein Genes	RNA Genes	Pseudogenes	Size (bp)
Chromosome 1	5541	5490	51	67	6,636,827
Plasmid 1	29	29	0	0	23,317
Total:	5570	5519	51	67	6,660,14

Pathways:	196
Enzymatic Reactions:	1186
Transport Reactions:	65
Polypeptides:	5452
Protein Complexes:	17
Enzymes:	980
Transporters:	98
Compounds:	823
Transcription Units:	3686
tRNAs:	5

Mycobacterium ulcerans Agy99

Replicon	Total Genes	Protein Genes	RNA Genes	Pseudogenes	Size (bp)
Chromosome 1	4981	4931	50	771	5,631,606
Plasmid 1	81	81	0	0	174,155
Total:	5062	5012	50	771	5,805,761

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Mycobacterium ulcerans strain Agy99 and its 174-kb pMUM001 plasmid

athways:	196
Enzymatic Reactions:	1209
Transport Reactions:	53
Polypeptides:	4241
Protein Complexes:	11
Enzymes:	841
Transporters:	67
Compounds:	806
Transcription Units:	3542
tRNAs:	51

Comparison

Organism	M. marinum M	M. ulcerans Agy99
Chromosomes	1	1
Organelle Chromosomes	0	0
Plasmids	1	1
Contigs	0	0
Genes	5,503	4,291
___ Genes of known or predicted molecular function	1,056	906
___ Genes with Exp Evidence	0	0
Protein Features	0	0
Protein Complexes	17	11
Pathways	236	232
___ Pathways with Exp Evidence	0	0
Metabolic Reactions	1,136	1,145
Transport Reactions	60	48
Compounds	822	805
Regulatory Interactions	0	0
Transcription Units	3,686	3,542
Promoters	0	0
Transcription Factor Binding Sites	0	0
Publications	849	852

Genes/Proteins	M. marinum M	M. ulcerans Agy99
Genes	5,503	4,291
___ Protein-coding Genes	5,452	4,241
___ tRNA Genes	52	51
___ rRNA Genes	3	3
___ tmRNA Genes	0	0
___ snRNA Genes	0	0
___ Regulatory RNA Genes	0	0
___ Other RNA Genes	2	2
Polypeptides	5,452	4,241
Protein Complexes	17	11
___ Heteromultimeric Protein Complexes	17	11
___ Homomultimeric Protein Complexes	0	0
Pseudogenes	67	771

Gene Annotation Status	M. marinum M	M. ulcerans Agy99
Genes of known or predicted molecular function	1,056	906
___ Genes of known molecular function	0	0
___ Genes of predicted molecular function	1,056	906
Genes of unknown molecular function	4,447	3,385

Enzymes	M. marinum M	M. ulcerans Agy99
Enzymes	843	730
___ Enzymes that catalyze Small Molecule reactions	733	613
___ Enzymes with a Physiologically Relevant Activator	0	0
___ Enzymes with a Physiologically Relevant Inhibitor	0	0
___ Enzymes with a Cofactor or Prosthetic Group	0	0

Transporters	M. marinum M	M. ulcerans Agy99
Uptake transporters	96	64
Efflux transporters	0	0
Transporters assigned to transport reactions	97	66
Genes assigned to transport proteins	122	81
Genes classified in MultiFun as transport genes	229	130