"If phiMU01 and phiMU02 prophages were mycobacterium phages probably they have some common features with Phage integrase of L5 and D29 mycobacterium phages ".
If this hypotesis is true in that case I can hope against hope.
This analysis will reinforce my conviction about the presence in the soil , fresch water or in other sources of specific phages for Mycobacterium ulcerans. I am sure "nothing seek, nothing find".
By Artemis software I have extracted :
-phiMU01 and phiMU02 prophage integrase sequences from Mycobacterium ulcerans genome.
>misc_feature misc_feature Prophage phiMU01 523696:542119 forward
CCTTGCCGATAGACGGTACCGGCGCGCCCTGACGGGA
CGCGAACGATCAAGAAGCTACCCGCGCCGGTGTCGCT
GGACGGCACTCTAATAACGTCGCGGCTCGCTGGCGTT
GGAATTCAG..........AATC
MUL 0529
>misc_feature misc_feature Prophage phiMU02 3582899:3606951 reverse
GTCAAGTGGTCGCAGGTTCAAATCCTGTCAGCCCGACCA
GAACGTTCTTACTCAAACCAGTGACCGAAAAGACACCGG
CCAAGGTGAGCGACTCCGTTCCGGTGGATCTAGGAGCC
CCTG......ACAT
MUL 3230
-phage integrase sequences from L5 and D29 Mycobacterium phage genome .
L5p33
D29p32
L5p33 DNA/D29p32 Dna Dot plot
MUL 0529 DNA / MUL 3230 DNA Dot plot
MUL 3230 Protein / MUL 0529 Protein Dot plot
MUL 0529 DNA / L5p33 DNA Dot plot
MUL 0529 Protein / L5p33 Protein Dot plot
MUL 0529 Protein / D29p32 Protein Dot plot
MUL 3230 DNA / L5p33 DNA Dot plot
MUL 3230 Protein / L5p33 Protein Dot plot
Is this hypotesis true ? In that case I can hope against hope.
Yes !!!
DISTANCE
D29p32 and MUL 0529 alignment
L5p33 and MUL 0529 alignment
Integrase and genomes GC Skew
