How to identify Nucleic Acid binding Motifs in Protein Sequences

L5 mycobacterium phage genome is used for example:

1-download the L5 mycobacterium phage genome (file.gbk from NCBI) or your genome (file.gbk).

2-open this file by Artemis software ( open source) or by another software.

3- each time select from Artemis software one gene and copy the corresponding protein sequence .

4-paste and filter this sequence in Sequence Manipulation Suite ( open source) if is necessary and copy this filtrate protein sequence .

5- open mJemboss software (open source) and paste the sequence in Helix-Turn-Helix program.

7-safe the result.

8 - in Jemboss by the same sequence open other programs.

9- align the sequences by Strap software (open source) or by Jalview software (open source) and plot the Tree.

L5p71(c44882-44331, 183Aa)


L5p36 (c26727-26557, 56Aa)

L5p44(c30476-28689, 595Aa)

L5p46(c31389-30961,142 Aa)

In L5 mycobacterium phage proteome are present these Helix-turn-helix motifs :

TREE





































Alignment