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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Thursday, 24 December 2009

Which is the Phage receptor? Third part.

Third comparison:BXZ2 phage genome

The Third comparison is with BXZ2 phage genome:

1- this is the result of Local (Smith-Waterman) comparison between the whole BXZ2 sequence and Proteins of Mycobacterium ulcerans Agy99:



By BugView:
4208 comparisons for user's query sequence and Mycobacterium ulcerans Agy99.
Finished in 4376 seconds.

Highest score = 1516 for cds_3629 (118619508)
Second score = 1108 for cds_967 (118616873)
Third score = 979 for cds_3641 (118619519)



2
-I have compared ,one by one, the indicated M.ulcerans cds with the
BXZ2 phage genome:


1°cds_3629


cds_3629 is (MUL_4367):

PE-PGRS family protein (Mycobacterium ulcerans Agy99)
Other Aliases: MUL_4367
Annotation: NC_008611.1 (4845511..4848744, complement)
GeneID: 4550406


By BugView:
89 comparisons for cds_3629 (118619508)
Finished in 1 seconds.

Highest score = 406 for cds_3 (29566025)
Second score = 247 for cds_4 (29566026)
Third score = 110 for cds_26 (29566045)

cds_3

Bxz2_gp4
gp4 [Mycobacterium phage Bxz2]
Other Aliases: Bxz2_gp4, Bxz2p04
Annotation: NC_004682.1 (1692..2726)
GeneID: 1259216


Accession.......... GeneID

NP_817595.11259216





I have compared , the indicated cds 3 with the M.ulcerans genome:

By BugView:

4208 comparisons for cds_3 (29566025)
Finished in 46 seconds.

Highest score = 414 for cds_3076 (118618968)
Second score = 413 for cds_620 (118616526)

Third score = 406 for cds_3629 (118619508)





2°cds_967

By BugView:
89 comparisons for cds_967 (118616873)
Finished in 0 seconds.

Highest score = 281 for cds_3 (29566025)
Second score = 212 for cds_4 (29566026)
Third score = 111 for cds_26 (29566045)







3°cds_3641


By BugView:

89 comparisons for cds_3641 (118619519)
Finished in 1 seconds.

Highest score = 383 for cds_3 (29566025)
Second score = 208 for cds_4 (29566026)
Third score = 118 for cds_26 (29566045)






In the cluster is present the gp5 from cds 4. But the gp4 from cds 3 is correlated to the gp5.

Accession................. GeneID

NP_817596.11259217