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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Thursday, 24 December 2009

Which is the Phage receptor? Second part.




Second comparison
:L5 phage genome


The second comparison is with L5 phage genome:

1- this is the result of Local (Smith-Waterman) comparison between the whole L5 sequence and Proteins of Mycobacterium ulcerans Agy99:


By BugView:
4208 comparisons for user's query sequence and Mycobacterium ulcerans Agy99.Finished in 4126 seconds.

Highest score = 1559 for
cds_3629 (118619508)
Second score = 1108 for
cds_2549 (118618448)
Third score = 993 for
cds_3641 (118619519)


2-I have compared ,one by one, the indicated M.ulcerans cds with the L5 genome:


1°cds_3629
cds_3629 is (MUL_4367):

PE-PGRS family protein (Mycobacterium ulcerans Agy99)
Other Aliases: MUL_4367
Annotation: NC_008611.1 (4845511..4848744, complement)
GeneID: 4550406

By BugView:
88 comparisons for cds_3629 (118619508)
Finished in 1 seconds.

Highest score = 244 for cds_31 (9625462)
Second score = 126 for cds_27 (9625458)
Third score = 104 for cds_9 (9625440



cds_31

L5p32
gene 32 [Mycobacterium phage L5]
Other Aliases: L5p32
Other Designations: predicted 21.3kd protein
Annotation: NC_001335.1 (23536..24198)
GeneID: 2942918

Accession............... GeneID.

NP_039696.12942918




I have compared , the indicated cds 31 with the M.ulcerans genome:



By
BugView
:
4208 comparisons for cds_31 (9625462).
Finished in 37 seconds.

Highest score = 264 for cds_3679 (118619555)
Second score = 261 for cds_3560 (118619440)
Third score = 261 for cds_2695 (118618593)





2°cds_2549




By BugView:
88 comparisons for cds_2549 (118618448)
Finished in 0 seconds.
Highest score = 234 for cds_31 (9625462)
Second score = 119 for cds_30 (9625461)
Third score = 107 for cds_27 (9625458)




3°cds_3641

By BugView:
88 comparisons for cds_3641 (118619519)
Finished in 1 seconds.

Highest score = 234 for cds_31 (9625462)
Second score = 111 for cds_11 (9625442)
Third score = 109 for cds_27 (9625458)