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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Thursday 26 November 2009

CHAOS Game by Emboss : D29, L5, BXZ2 and TM4 mycobacteriophages


Chaos
creates a chaos game representation (CGR) plot for a nucleotide sequence. A CGR plot represents a nucleotide sequence as a square box with an A, G, C, or T nucleotide at each corner.

The box contains dots, each one representing a dinucleotide. All overlapping dinucleotides from the start to the end of the sequence are plotted.

Regions which are devoid of dots (or heavily covered with dots) indicate short sequence motifs that are unusually infrequent (or frequent).

CGR plots depict base composition and sequentiality and is a unique visual representation of a sequence that complements more traditional linear representations.

Algorithm


The plot is generated as follows. A box is drawn and an A, G, C, or T is drawn at each corner. Starting from the middle, move half way to the corner of the box representing the first base in the sequence and draw a dot. Then for each subsequent base move half way to the corresponding box corner and draw a dot. Finally display the number and percentage values of AGCT bases. The result is an image of a square sprinkled with dots.

D29


L5

Bxz2
TM4