From the only information for growing Phages on Mycobacterium ulcerans

From:
"Host range of 14 mycobacteriophages in Mycobacterium ulcerans and ..."

"D29 seems to be a good choice for mycobacteriophage assays for the detection of M. ulcerans in patient samples or to investigate the drug resistance of mycobacterial isolates. This phage proved to be lytic in M.ulcerans species which originated from four different continents.

M. ulcerans produces the polyketide-derived macrolide mycolactone, which is required for the tissue damage that characterizes Buruli ulcer (George et al., 1999; Mve-Obianget al., 2003). Although mycolactone is a major component of the cell surface of M. ulcerans, it does not seem to be involved in the binding of phages or the injection of phage DNA, since the avirulent mycolactone-negative mutant 1615M showed the same host range as the wild-type strain.

In summary, we have shown that the mycobacteriophages D29, Bxz2, L5 and TM4 have a broad, and to some extent similar, host range among the slow-growing mycobacteria."


I have compared the genomes of this phages by MAUVE Software, GATA Software and GEPARD Software.

L5 ,D29 and Bxz2 are close ,while TM4 is not close to this phage group but it is distant.

Alignment by Mauve: D29,Bxz2,L5 and TM4

Alignment by Mauve: D29,Bxz2,L5

Alignment by Mauve: D29,TM4

Alignment by Mauve:Bxz2,TM4

Alignment by Mauve: L5,TM4

Alignment by Mauve: D29,L5

Alignment by Mauve: D29, Bxz2

Alignment by Mauve: Bxz2,L5

Alignment by Gata: D29,L5


Alignment by Gata: D29,Bxz2

Alignment by Gata: Bxz2,L5

Alignment by Gata: Bxz2,TM4

Alignment by Gata: L5,TM4

Alignment by Gata: D29,TM4

Dot Plot L5 and TM4 by Gepard

Dot Plot D29 and TM4 by Gepard

Dot Plot Bxz2 and TM4 by Gepard

Dot Plot D29 and Bxz2 by Gepard

Dot Plot L5 and Bxz2 by Gepard

Working hypothesis on Receptors

If all these phages are growing on Mycobaterium smegmatis MC2 155 and on Mycobacterium ulcerans must have the same receptor or different receptors.



Phage...M.ulcerans Receptor (R1) ...M.smegmatis Receptor(R2)

L5................... R1L5..................................... R2L5


D29................. R1D29................................... R2D29


Bxz2................ R1Bxz2.................................. R2Bxz2


TM4 .................R1TM4.................................... R2TM4


Suppositions:

1)R1L5,R1D29,R1Bxz2,R1TM4 are the same,
or
R1L5,R1D29,R1Bxz2,R1TM4 are different one another.



2)R2L5,R2D29,R2Bxz2,R2TM4 are the same,
or
R2L5,R2D29,R2Bxz2,R2TM4 are different one another.


3)R1L5,R1D29,R1Bxz2,R1TM4 and R2L5,R2D29,R2Bxz2,R2TM4 are the same,
or
R1L5,R1D29,R1Bxz2,R1TM4,R2L5,R2D29,R2Bxz2,R2TM4 are different one another.