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"In silico"


 From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
 Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Wednesday, 30 September 2009

D29, L5, Bxz2 and TM4 mycobacteriophages


I have investigated the genomes of these phages searching for a relationship of cause and effect (D29,Bxz2,L5 and TM4 phages grow on M.ulcerans and M.smegmatis).
Each time two genomes are compared by BugView software ( Smith Waterman algorithm ).The results obtained are(cds from files .gbk):


D29 on L5:31,39,85/on BXZ2:3,4,38/on TM4:24,27,39

L5 on D29:34,39,82 /on BXZ2:3,4,38/on TM4:24,27,39

BXZ2 on D29:34,39,51/on L5:31,39,85/on TM4:24,27,39

TM4 on D29:34,39,80/on L5:31,39,85/on BXZ2:4,2,38




I have selected ( Sockeye software):

cds 4 from Bxz2 genome

cds 24 from TM4 genome



cds 31 from L5 genome


cds 34 from D29 genome










Posted by Rodolfo Berretti at 00:25
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