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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Friday 6 February 2009

The Work Flow

Work Flow


a-
Patient has a Bacterial infection.

b-In this infection
Bacteria are Drug- Resistant.

c-
Recovery chance is linked both to the Infection Site and to the Efficacy gradient of Phage therapy:

-
Skin infections,external infections and infections into accessible Anatomical Cavities.
-
Intestinal infections.
-
Infection Sites attacked only by Phages carried in situ by bloodstream.

d-A
broad spectrum Phage Preparation, containing different types of Phages,is used.

e-Phage Preparation is administrated,in liquid form ,spreads directly on to
surfaces of the body or topically on infected wounds or into the anatomical cavities or orally.

f-
Bacteria are isolated from the infection site and all microbiological tests must be start soon after.

g-
Bacteria are tested by this Phage Preparation for checking its lytic effects.

h- If this
Phage Preparation is not fully active, a new Phage Preparation must be prepared:see What is an autoPhage?

Doubtful Point

From this flow an important key question emerges about a doubtful point:

Does Oral Phage administration works?

The
answer may change the potentiality of Phage Therapy.