Genome sequence of enterohaemorrhagic Escherichia coli O157:H7
Escherichia coli O157:H7 EDL933 Genome
Genome Properties Report
Genes assigned a role category
Organism Gene List
PFGE method
Organism-------------------Escherichia coli
Recommended lysis enzyme--proteinase K
Restriction enzyme---------XbaI,NotI,SfiI
Approximate no. of--15–20,12–15,15–20
restriction Fragments
Fragments size-10–500,10–1000,10–700
range (kb)
General information
E.coli (CDA)
E.coli (FDA)
Identification of Enterobacteriaceae
E.coli 0157
Escherichia coli can be classified by their characteristic virulence properties and different mechanisms that cause disease.
A. Enteroaggregative E. coli (EAEC)
B. Enterohemorrhagic E. coli (EHEC)
C. Enteroinvasive E. coli (EIEC)
D. Enteropathogenic E. coli (EPEC)
E. Enterotoxigenic E. coli (ETEC)
All of the above classes of E. coli cause diarrhea.However, only EHEC produce a verotoxin that has many properties that are similar to Shiga toxin.
EHEC has been associated with hemorrhagic colitis,a severe form of diarrhea, and with HUS. HUS is a disease resulting in acute renal failure, microangiopathic hemolytic anemia, and thrombocytopenia.
Escherichia coli 0157:H7 strains are classically associated with outbreaks of diarrhea after ingestion of undercooked hamburger at fastfood restaurants. Many cases of hemorrhagic colitis and its associated complications can be prevented by thoroughly cooking ground beef.The other organisms listed can cause GI disturbances;
however, E. coli is the classic disturbing pathogen in this case.
