Complete Genome Sequence Klebsiella pneumoniae 342
Klebsiella pneumoniae Kp342 Genome
Genome Properties Report
Genes assigned a role category
Organism Gene List
PFGE method
Organism-------------------Klebsiella spp.
Recommended lysis enzyme--proteinase K
Restriction enzyme---------XbaI
Approximate no. of---------15–20
restriction Fragments
Fragments size------------10–700
range (kb)
General information
The genus Klebsiella, which belongs to the includes family Enterobacteriaceae five species,with the most clinically significant being K. pneumoniae.
Klebsiella pneumoniae is a large, nonmotile, with a prominent polysaccharide gram-negative rod capsule.
The capsule is antiphagocytic and retards leukocyte migration into an infected area.
Another virulence factor of K. pneumoniae is its propensity to develop resistance to multiple antibiotics.All strains of K. pneumoniae are innately resistant to ampicillin, because of the production of β-lactamase.
Acquisition of resistance to other antibiotics usually occurs by transfer of plasmids from other organisms.Recently strains of nosocomially acquired K.pneumoniae have been isolated that produce an extended spectrum β-lactamase and therefore are resistant to all β-lactam antibiotics.
Klebsiella pneumoniae will grow rapidly producing large mucoid colonies on routine laboratory media.Colonies are often extremely mucoid and will tend to drip into the lid of the plate while incubating in an inverted position. Pink colonies will be evident on MacConkey agar indicating their fermentation of lactose.
Both K. oxytoca and especially K. rhinoscleromatis are slower growing than
K. pneumoniae and the other Enterobacteriaceae. All Klebsiella species are very closely related with nearly identical biochemical reactions, except for the fact that K. pneumoniae is indole negative, and K. oxytoca is indole positive.
Treatment of K. pneumoniae pneumonia would be based on the susceptibility of the isolate. Treatment can be complicated by the presence of multidrug-resistant strains.Most strains are susceptible to extended spectrum cephalosporins such as cefepime as well as fluoroquinolones such as moxifloxacin. In cases of strains that produce an extended spectrum β-lactamase, the treatment of choice would be imipenem or meropenem.
Prevention of spread in the hospital would involve appropriate infection control procedures to isolate patients with multidrug-resistant organisms. Klebsiella oxytoca has similar susceptibility patterns as K. pneumoniae and can also produce extended spectrum β-lactamases.
Klebsiella pneumoniae is a large, nonmotile, with a prominent polysaccharide gram-negative rod capsule.
The capsule is antiphagocytic and retards leukocyte migration into an infected area.
Another virulence factor of K. pneumoniae is its propensity to develop resistance to multiple antibiotics.All strains of K. pneumoniae are innately resistant to ampicillin, because of the production of β-lactamase.
Acquisition of resistance to other antibiotics usually occurs by transfer of plasmids from other organisms.Recently strains of nosocomially acquired K.pneumoniae have been isolated that produce an extended spectrum β-lactamase and therefore are resistant to all β-lactam antibiotics.
Klebsiella pneumoniae will grow rapidly producing large mucoid colonies on routine laboratory media.Colonies are often extremely mucoid and will tend to drip into the lid of the plate while incubating in an inverted position. Pink colonies will be evident on MacConkey agar indicating their fermentation of lactose.
Both K. oxytoca and especially K. rhinoscleromatis are slower growing than
K. pneumoniae and the other Enterobacteriaceae. All Klebsiella species are very closely related with nearly identical biochemical reactions, except for the fact that K. pneumoniae is indole negative, and K. oxytoca is indole positive.
Treatment of K. pneumoniae pneumonia would be based on the susceptibility of the isolate. Treatment can be complicated by the presence of multidrug-resistant strains.Most strains are susceptible to extended spectrum cephalosporins such as cefepime as well as fluoroquinolones such as moxifloxacin. In cases of strains that produce an extended spectrum β-lactamase, the treatment of choice would be imipenem or meropenem.
Prevention of spread in the hospital would involve appropriate infection control procedures to isolate patients with multidrug-resistant organisms. Klebsiella oxytoca has similar susceptibility patterns as K. pneumoniae and can also produce extended spectrum β-lactamases.
