Mycobacteriophage Genome Restriction Analysis

D29

NC_001900.gbk, 49136 bp. (LINEAR)

Restriction analysis 29/11/2009

pDRAW32 revision 1.1.101

Rebase containing 280 enzymes.

16 enzymes match enzyme selection criteria.

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AatII (G_ACGT'C) [ZraI]
Cuts 1 time.
Cuts at position 26529.
Fragment sizes 26529, 22607.

AfeI (AGC'GCT) [Eco47III,Aor51HI]
Cuts 1 time.
Cuts at position 19096.
Fragment sizes 19096, 30040.

AflII (C'TTAA_G) [BfrI,BspTI,Bst98I,BstAFI,MspCI,Vha464I]
Cuts 1 time.
Cuts at position 26904.
Fragment sizes 26904, 22232.

AseI (AT'TA_AT) [VspI,PshBI]
Cuts 1 time.
Cuts at position 229.
Fragment sizes 229, 48907.

EcoRI (G'AATT_C)
Cuts 1 time.
Cuts at position 35257.
Fragment sizes 35257, 13879.

HpaI (GTT'AAC) [KspAI]
Cuts 1 time.
Cuts at position 4252.
Fragment sizes 4252, 44884.

MfeI (C'AATT_G) [MunI]
Cuts 2 times.
Cuts at positions 4345, 33476.
Fragment sizes 4345, 29131, 15660.

SacII (CC_GC'GG) [Cfr42I,KspI,Sfr303I,SgrBI,SstII]
Cuts 2 times.
Cuts at positions 7970, 32235.
Fragment sizes 7970, 24265, 16901.

SgrDI (CG'TCGA_CG)
Cuts 1 time.
Cuts at position 47838.
Fragment sizes 47838, 1298.

SmaI (CCC'GGG) [Cfr9I,TspMI,XmaI,XmaCI]
Cuts 2 times.
Cuts at positions 23175, 36011.
Fragment sizes 23175, 12836, 13125.

SpeI (A'CTAG_T) [AhlI,BcuI]
Cuts 2 times.
Cuts at positions 38318, 48289.
Fragment sizes 38318, 9971, 847.

SrfI (GCCC'GGGC)
Cuts 1 time.
Cuts at position 23175.
Fragment sizes 23175, 25961.

StuI (AGG'CCT) [AatI,Eco147I,PceI,SseBI]
[dcm methylated]
Cuts 2 times.
Cuts at positions 23559, 45643.
Fragment sizes 23559, 22084, 3493.

XbaI (T'CTAG_A)
[dam methylated]
Cuts 1 time.
Cuts at position 47494.
Fragment sizes 47494, 1642.

XmaI (C'CCGG_G) [SmaI,Cfr9I,TspMI,XmaCI]
Cuts 2 times.
Cuts at positions 23173, 36009.
Fragment sizes 23173, 12836, 13127.

ZraI (GAC'GTC) [AatII]
Cuts 1 time.
Cuts at position 26527.
Fragment sizes 26527, 22609.

L5

NC_001335.gbk, 52297 bp. (LINEAR)
Restriction analysis 29/11/2009
pDRAW32 revision 1.1.101
Rebase containing 280 enzymes.
21 enzymes match enzyme selection criteria.

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AatII (G_ACGT'C) [ZraI]
Cuts 2 times.
Cuts at positions 23726, 40679.
Fragment sizes 23726, 16953, 11618.

AfeI (AGC'GCT) [Eco47III,Aor51HI]
Cuts 1 time.
Cuts at position 6414.
Fragment sizes 6414, 45883.

AflII (C'TTAA_G) [BfrI,BspTI,Bst98I,BstAFI,MspCI,Vha464I]
Cuts 1 time.
Cuts at position 15905.
Fragment sizes 15905, 36392.

ApoI (r'AATT_y) [AcsI,XapI]
Cuts 2 times.
Cuts at positions 44065, 48703.
Fragment sizes 44065, 4638, 3594.

BbeI (G_GCGC'C) [NarI,DinI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 14171.
Fragment sizes 14171, 38126.

Bsu36I (CC'TnA_GG) [AxyI,Bse21I,Eco81I]
Cuts 2 times.
Cuts at positions 12530, 24833.
Fragment sizes 12530, 12303, 27464.

HpaI (GTT'AAC) [KspAI]
Cuts 1 time.
Cuts at position 49185.
Fragment sizes 49185, 3112.

KasI (G'GCGC_C) [NarI,BbeI,DinI,EgeI,EheI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 14167.
Fragment sizes 14167, 38130.

MreI (CG'CCGG_CG)
Cuts 1 time.
Cuts at position 16616.
Fragment sizes 16616, 35681.

NarI (GG'CG_CC) [BbeI,DinI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 14168.
Fragment sizes 14168, 38129.

PasI (CC'CwG_GG)
Cuts 2 times.
Cuts at positions 26309, 43433.
Fragment sizes 26309, 17124, 8864.

PmlI (CAC'GTG) [PmaCI,AcvI,BbrPI,Eco72I,PspCI]
Cuts 2 times.
Cuts at positions 37771, 41672.
Fragment sizes 37771, 3901, 10625.

PvuI (CG_AT'CG) [BpvUI,MvrI,Ple19I]
Cuts 1 time.
Cuts at position 959.
Fragment sizes 959, 51338.

PvuII (CAG'CTG)
Cuts 2 times.
Cuts at positions 24091, 42988.
Fragment sizes 24091, 18897, 9309.

RsrII (CG'GwC_CG) [CpoI,CspI,Rsr2I]
Cuts 1 time.
Cuts at position 16814.
Fragment sizes 16814, 35483.

SacII (CC_GC'GG) [Cfr42I,KspI,Sfr303I,SgrBI,SstII]
Cuts 2 times.
Cuts at positions 31711, 52194.
Fragment sizes 31711, 20483, 103.

SfiI (GGCCn_nnn'nGGCC)
[dcm methylated]
Cuts 2 times.
Cuts at positions 11292, 39562.
Fragment sizes 11292, 28270, 12735.

SfoI (GGC'GCC) [NarI,BbeI,DinI,EgeI,EheI,KasI,Mly113I]
Cuts 1 time.
Cuts at position 14169.
Fragment sizes 14169, 38128.

SpeI (A'CTAG_T) [AhlI,BcuI]
Cuts 2 times.
Cuts at positions 5596, 37850.
Fragment sizes 5596, 32254, 14447.

XbaI (T'CTAG_A)
[dam methylated]
Cuts 2 times.
Cuts at positions 3150, 36267.
Fragment sizes 3150, 33117, 16030.

ZraI (GAC'GTC) [AatII]
Cuts 2 times.
Cuts at positions 23724, 40677.
Fragment sizes 23724, 16953, 11620.

Bxz2






TM4