Monday, 10 August 2009

How to explore a Phage "In Silico": Mycobacterium phage D29 . Step 1




All steps are the same for all phages.




*Mycobacterium phage D29


(dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae; L5-like viruses)



*
Go to
DBGET Search
write : Mycobacterium phage D29
open and the Page is showing:

*In DNA sequence open NCBI and the complete genome is ready with CDS and the relative sequences in amino acids.

*In KEGG Vgenome open
NC_001900
and the page is ready with :

  Number of nucleotides:         49136

Number of protein genes: 79

Number of RNA genes: 5

G+C content: 63.5%




*Go to ITVC index viruses for "L5 -like viruses"
and see the other members and Mycobacterium phage L5.


*Go to NCBI Map, select Virus. The Virus page is open , select in the window Siphoviridae, open and all phage genomes of this Family are showing.



*Go to EBI Genomes write : Mycobacterium phage D29 and open.
The page with concise data is showing.


*Go to HAMAP proteomes and write in the upper window : Mycobacterium phage D29 and open.
The page with genes and proteins is showing.



*Go to Genome online database
and in the Google window write: Mycobacterium phage D29 , select web data and open. All data on Mycobacteriophage D29 are showing.