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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Wednesday, 15 February 2012

Indian TB cases 'can't be cured' ( but DS6A phage could be used)


 DS6A phage (my old idea)

From this scientific work :
" In 1981 Sula et al. reported positive indicators
with a reduction in the observed lesions in the spleen, lungs and livers of guinea pigs following therapy with DS-6A. More recent work demonstrated that phage therapy could have a beneficial effect in guinea pigs with disseminated tuberculosis, but that its action
was considerably less pronounced than that of isoniazid monotherapy."


From this scientific work
"Sula et al. [63] infected guinea pigs with M. tuberculosis and then treated them with subcutaneous injections of three different bacteriophages twice weekly for 10 weeks.
One of these bacteriophages, designated DS-6A, produced an antibacterial effect at least as good as isoniazid."



From this scientific work:
"In addition, the previously isolated phage DS6A, the only one of the 138 phages that does not infect M. smegmatis was sequenced and annotated." 


From this scientific work: