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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Saturday 21 August 2010

Going in search of phages for Mycobacterium ulcerans

This is my opinion about the question.

In practice at the moment I do not have the possibility to search phages for Mycobacterium ulcerans but, if I do not have real phages, however, I can study all potential phages (prophages) inside Mycobacterium marinum and Mycobacterium ulcerans genome.


1-If I find some correlations among "prophages" of these Mycobacteria I have further evidence that M. ulcerans is originated from M.marinum.

2-
If I find some correlations between "prophages" of these Mycobacteria I have further evidence that specific phages for M. ulcerans and M.marinum could exist.



I quote the passage from:


Insights from the complete genome sequence of
Mycobacterium marinum on the evolution of Mycobacterium tuberculosis


"...and 10 putative prophages, named phiMmar01–10 (Supplemental Table 2)."




I will study these potential "prophages" one by one.