Identification and Characterization of IS2404 and IS2606:
Two Distinct Repeated Sequences for Detection of Mycobacterium ulcerans by PCR
TIMOTHY STINEAR, C. ROSS, JOHN K. DAVIES, LUI MARINO,ROY M. ROBINS-BROWNE, OPPEDISANO, AINA SIEVERS,AND PAUL D. R.
“IS2404 was identified by complete sequencing of a previously described repetitive DNA segment from M. ulcerans. This element is 1,274 bp long, contains 12-bp inverted repeats and a
single open reading frame (ORF) potentially encoding a protein of 327 amino acids (aa),and apparently generates 7-bp direct repeats upon transposition.”
By Mycobacterium ulcerans genome and using UGENE software it is possible to copy IS 2404 sequence.
By FastPCR software it is possible :
1 – to apply the WHO primers.
or
2- to apply the primers generated by FastPCR software.
