At the moment my answer to this question is negative.
Mostly well-known mycobacteriopages are isolated and multiplied by growth on Mycobacterium smegmatis lawn and are also lysogenic phages:
Mycobacterium phage 244
Mycobacterium phage Adjutor
Mycobacterium phage Angel
Mycobacterium phage BPs
Mycobacterium phage Barnyard
Mycobacterium phage Bethlehem
Mycobacterium phage Boomer
Mycobacterium phage Brujita
Mycobacterium phage Butterscotch
Mycobacterium phage Bxb1
Mycobacterium phage Bxz1
Mycobacterium phage Bxz2
Mycobacterium phage Cali
Mycobacterium phage Catera
Mycobacterium phage Chah
Mycobacterium phage Che12
Mycobacterium phage Che8
Mycobacterium phage Che9c
Mycobacterium phage Che9d
Mycobacterium phage Cjw1
Mycobacterium phage Cooper
Mycobacterium phage Corndog
Mycobacterium phage D29
Mycobacterium phage DD5
Mycobacterium phage Fruitloop
Mycobacterium phage Giles
Mycobacterium phage Gumball
Mycobacterium phage Halo
Mycobacterium phage Jasper
Mycobacterium phage KBG
Mycobacterium phage Konstantine
Mycobacterium phage Kostya
Mycobacterium phage L5
Mycobacterium phage Llij
Mycobacterium phage Lockley
Mycobacterium phage Myrna
Mycobacterium phage Nigel
Mycobacterium phage Omega
Mycobacterium phage Orion
Mycobacterium phage PBI1
Mycobacterium phage PG1
Mycobacterium phage PLot
Mycobacterium phage PMC
Mycobacterium phage Pacc40
Mycobacterium phage Phaedrus
Mycobacterium phage Phlyer
Mycobacterium phage Pipefish
Mycobacterium phage Porky
Mycobacterium phage Predator
Mycobacterium phage Pukovnik
Mycobacterium phage Qyrzula
Mycobacterium phage Ramsey
Mycobacterium phage Rizal
Mycobacterium phage Rosebush
Mycobacterium phage ScottMcG
Mycobacterium phage Solon
Mycobacterium phage Spud
Mycobacterium phage TM4
Mycobacterium phage Troll4
Mycobacterium phage Tweety
Mycobacterium phage U2
Mycobacterium phage Wildcat
To find "genuine" lytic mycobacteriophages is not easy and it seems to me that it is also rare .
D29 is a lytic phage but I can not consider this phage a "genuine " lytic phage by genome analysis.
These mycobacteriophages have a broad Host range and this feature is not favourable when they are used in Phage therapy.
I am of the opinion that Mycobacterium marinum may be the valid alternative to Mycobacterium smegmatis for growing and isolating phages with a potential action on Mycobacterium ulcerans but until now there is little information about growing phages on Mycobacterium marinum.
It is a virgin ground and it is indispensable to set to work on.
