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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Monday, 19 October 2009

Codons of TM4 , Bxz2, L5 and D29 mycobacteriophages



D29 Codons


Codon Usage results
Results for 49136 residue sequence "gi|9630386|ref|NC_001900.1|
Mycobacterium phage D29, complete genome" starting "GGTCGGTTAT"

AmAcid Codon Number /1000 Fraction

Ala GCG 407.00 24.85 0.28
Ala GCA 273.00 16.67 0.19
Ala GCT 361.00 22.04 0.25
Ala GCC 422.00 25.77 0.29

Cys TGT 179.00 10.93 0.42
Cys TGC 247.00 15.08 0.58

Asp GAT 276.00 16.85 0.43
Asp GAC 368.00 22.47 0.57

Glu GAG 278.00 16.97 0.54
Glu GAA 241.00 14.71 0.46

Phe TTT 42.00 2.56 0.14
Phe TTC 251.00 15.33 0.86

Gly GGG 274.00 16.73 0.19
Gly GGA 299.00 18.26 0.21
Gly GGT 370.00 22.59 0.26
Gly GGC 487.00 29.74 0.34

His CAT 167.00 10.20 0.39
His CAC 262.00 16.00 0.61

Ile ATA 30.00 1.83 0.09
Ile ATT 38.00 2.32 0.11
Ile ATC 264.00 16.12 0.80

Lys AAG 208.00 12.70 0.84
Lys AAA 39.00 2.38 0.16

Leu TTG 198.00 12.09 0.18
Leu TTA 17.00 1.04 0.02
Leu CTG 290.00 17.71 0.26
Leu CTA 56.00 3.42 0.05
Leu CTT 200.00 12.21 0.18
Leu CTC 338.00 20.64 0.31

Met ATG 155.00 9.46 1.00

Asn AAT 44.00 2.69 0.20
Asn AAC 178.00 10.87 0.80

Pro CCG 521.00 31.81 0.34
Pro CCA 362.00 22.10 0.24
Pro CCT 302.00 18.44 0.20
Pro CCC 328.00 20.03 0.22

Gln CAG 341.00 20.82 0.64
Gln CAA 191.00 11.66 0.36

Arg AGG 273.00 16.67 0.11
Arg AGA 197.00 12.03 0.08
Arg CGG 540.00 32.97 0.23
Arg CGA 532.00 32.48 0.22
Arg CGT 367.00 22.41 0.15
Arg CGC 490.00 29.92 0.20

Ser AGT 136.00 8.30 0.07
Ser AGC 318.00 19.42 0.17
Ser TCG 534.00 32.60 0.29
Ser TCA 277.00 16.91 0.15
Ser TCT 226.00 13.80 0.12
Ser TCC 359.00 21.92 0.19

Thr ACG 374.00 22.84 0.35
Thr ACA 182.00 11.11 0.17
Thr ACT 179.00 10.93 0.17
Thr ACC 335.00 20.45 0.31

Val GTG 283.00 17.28 0.30
Val GTA 143.00 8.73 0.15
Val GTT 172.00 10.50 0.18
Val GTC 350.00 21.37 0.37

Trp TGG 327.00 19.97 1.00

Tyr TAT 17.00 1.04 0.11
Tyr TAC 133.00 8.12 0.89

End TGA 242.00 14.78 0.73
End TAG 72.00 4.40 0.22
End TAA 16.00 0.98 0.05


L5 Codons



Codon Usage results
Results for 52297 residue sequence "gi|9625433|ref|NC_001335.1|
Mycobacterium phage L5, complete genome" starting "GGTCGGTTAT"

AmAcid Codon Number /1000 Fraction

Ala GCG 432.00 24.78 0.28
Ala GCA 288.00 16.52 0.19
Ala GCT 391.00 22.43 0.25
Ala GCC 429.00 24.61 0.28

Cys TGT 203.00 11.65 0.44
Cys TGC 259.00 14.86 0.56

Asp GAT 317.00 18.18 0.44
Asp GAC 402.00 23.06 0.56

Glu GAG 291.00 16.69 0.50
Glu GAA 293.00 16.81 0.50

Phe TTT 69.00 3.96 0.24
Phe TTC 220.00 12.62 0.76

Gly GGG 281.00 16.12 0.18
Gly GGA 331.00 18.99 0.22
Gly GGT 425.00 24.38 0.28
Gly GGC 501.00 28.74 0.33

His CAT 249.00 14.28 0.44
His CAC 313.00 17.96 0.56

Ile ATA 38.00 2.18 0.10
Ile ATT 52.00 2.98 0.14
Ile ATC 286.00 16.41 0.76

Lys AAG 162.00 9.29 0.76
Lys AAA 52.00 2.98 0.24

Leu TTG 186.00 10.67 0.14
Leu TTA 22.00 1.26 0.02
Leu CTG 319.00 18.30 0.25
Leu CTA 93.00 5.34 0.07
Leu CTT 261.00 14.97 0.20
Leu CTC 408.00 23.41 0.32

Met ATG 183.00 10.50 1.00

Asn AAT 49.00 2.81 0.20
Asn AAC 192.00 11.01 0.80

Pro CCG 518.00 29.72 0.33
Pro CCA 332.00 19.05 0.21
Pro CCT 362.00 20.77 0.23
Pro CCC 358.00 20.54 0.23

Gln CAG 376.00 21.57 0.59
Gln CAA 256.00 14.69 0.41

Arg AGG 286.00 16.41 0.11
Arg AGA 239.00 13.71 0.10
Arg CGG 523.00 30.00 0.21
Arg CGA 590.00 33.85 0.23
Arg CGT 409.00 23.46 0.16
Arg CGC 464.00 26.62 0.18

Ser AGT 182.00 10.44 0.10
Ser AGC 310.00 17.78 0.17
Ser TCG 466.00 26.73 0.25
Ser TCA 267.00 15.32 0.15
Ser TCT 284.00 16.29 0.16
Ser TCC 323.00 18.53 0.18

Thr ACG 309.00 17.73 0.32
Thr ACA 166.00 9.52 0.17
Thr ACT 177.00 10.15 0.18
Thr ACC 313.00 17.96 0.32

Val GTG 276.00 15.83 0.26
Val GTA 154.00 8.83 0.14
Val GTT 274.00 15.72 0.26
Val GTC 359.00 20.59 0.34

Trp TGG 328.00 18.82 1.00

Tyr TAT 40.00 2.29 0.21
Tyr TAC 150.00 8.60 0.79

End TGA 229.00 13.14 0.67
End TAG 86.00 4.93 0.25
End TAA 29.00 1.66 0.08


Bxz2 Codons



Codon Usage results
Results for 50913 residue sequence "gi|29566021|ref|NC_004682.1|
Mycobacterium phage Bxz2,complete genome" starting "CGGGTGGTAA"

AmAcid Codon Number /1000 Fraction

Ala GCG 514.00 30.29 0.31
Ala GCA 288.00 16.97 0.17
Ala GCT 375.00 22.10 0.23
Ala GCC 483.00 28.46 0.29

Cys TGT 189.00 11.14 0.42
Cys TGC 263.00 15.50 0.58

Asp GAT 267.00 15.73 0.41
Asp GAC 392.00 23.10 0.59

Glu GAG 296.00 17.44 0.57
Glu GAA 222.00 13.08 0.43

Phe TTT 37.00 2.18 0.13
Phe TTC 259.00 15.26 0.88

Gly GGG 322.00 18.97 0.21
Gly GGA 332.00 19.56 0.22
Gly GGT 378.00 22.27 0.25
Gly GGC 509.00 29.99 0.33

His CAT 193.00 11.37 0.41
His CAC 276.00 16.26 0.59

Ile ATA 28.00 1.65 0.09
Ile ATT 40.00 2.36 0.12
Ile ATC 260.00 15.32 0.79

Lys AAG 190.00 11.20 0.88
Lys AAA 27.00 1.59 0.12

Leu TTG 169.00 9.96 0.14
Leu TTA 10.00 0.59 0.01
Leu CTG 334.00 19.68 0.28
Leu CTA 79.00 4.66 0.07
Leu CTT 210.00 12.37 0.18
Leu CTC 376.00 22.16 0.32

Met ATG 160.00 9.43 1.00

Asn AAT 38.00 2.24 0.15
Asn AAC 209.00 12.32 0.85

Pro CCG 549.00 32.35 0.33
Pro CCA 354.00 20.86 0.21
Pro CCT 387.00 22.80 0.23
Pro CCC 380.00 22.39 0.23

Gln CAG 321.00 18.92 0.62
Gln CAA 195.00 11.49 0.38

Arg AGG 348.00 20.51 0.14
Arg AGA 214.00 12.61 0.09
Arg CGG 529.00 31.17 0.22
Arg CGA 506.00 29.82 0.21
Arg CGT 344.00 20.27 0.14
Arg CGC 489.00 28.82 0.20

Ser AGT 171.00 10.08 0.09
Ser AGC 330.00 19.45 0.18
Ser TCG 511.00 30.11 0.28
Ser TCA 256.00 15.09 0.14
Ser TCT 229.00 13.49 0.12
Ser TCC 341.00 20.09 0.19

Thr ACG 359.00 21.15 0.36
Thr ACA 162.00 9.55 0.16
Thr ACT 177.00 10.43 0.18
Thr ACC 295.00 17.38 0.30

Val GTG 298.00 17.56 0.30
Val GTA 125.00 7.37 0.13
Val GTT 208.00 12.26 0.21
Val GTC 357.00 21.04 0.36

Trp TGG 319.00 18.80 1.00

Tyr TAT 28.00 1.65 0.18
Tyr TAC 126.00 7.42 0.82

End TGA 243.00 14.32 0.72
End TAG 78.00 4.60 0.23
End TAA 16.00 0.94 0.05

TM4 Codons

Codon Usage results
Results for 52797 residue sequence "gi|18496886|ref|NC_003387.1|
Mycobacterium phage TM4, complete genome" starting "CTCGCGGCAT"

AmAcid Codon Number /1000 Fraction

Ala GCG 822.00 46.71 0.34
Ala GCA 435.00 24.72 0.18
Ala GCT 434.00 24.66 0.18
Ala GCC 698.00 39.66 0.29

Cys TGT 107.00 6.08 0.28
Cys TGC 281.00 15.97 0.72

Asp GAT 264.00 15.00 0.30
Asp GAC 616.00 35.00 0.70

Glu GAG 420.00 23.86 0.72
Glu GAA 164.00 9.32 0.28

Phe TTT 87.00 4.94 0.31
Phe TTC 194.00 11.02 0.69

Gly GGG 317.00 18.01 0.18
Gly GGA 172.00 9.77 0.10
Gly GGT 298.00 16.93 0.17
Gly GGC 946.00 53.75 0.55

His CAT 205.00 11.65 0.36
His CAC 360.00 20.46 0.64

Ile ATA 31.00 1.76 0.09
Ile ATT 82.00 4.66 0.25
Ile ATC 221.00 12.56 0.66

Lys AAG 207.00 11.76 0.73
Lys AAA 78.00 4.43 0.27

Leu TTG 81.00 4.60 0.09
Leu TTA 38.00 2.16 0.04
Leu CTG 413.00 23.47 0.44
Leu CTA 71.00 4.03 0.08
Leu CTT 69.00 3.92 0.07
Leu CTC 263.00 14.94 0.28

Met ATG 149.00 8.47 1.00

Asn AAT 80.00 4.55 0.26
Asn AAC 228.00 12.96 0.74

Pro CCG 647.00 36.76 0.46
Pro CCA 147.00 8.35 0.10
Pro CCT 246.00 13.98 0.17
Pro CCC 367.00 20.85 0.26

Gln CAG 319.00 18.13 0.45
Gln CAA 397.00 22.56 0.55

Arg AGG 200.00 11.36 0.07
Arg AGA 56.00 3.18 0.02
Arg CGG 645.00 36.65 0.23
Arg CGA 726.00 41.25 0.25
Arg CGT 344.00 19.55 0.12
Arg CGC 886.00 50.34 0.31

Ser AGT 88.00 5.00 0.08
Ser AGC 283.00 16.08 0.27
Ser TCG 432.00 24.55 0.41
Ser TCA 136.00 7.73 0.13
Ser TCT 56.00 3.18 0.05
Ser TCC 69.00 3.92 0.06

Thr ACG 366.00 20.80 0.41
Thr ACA 134.00 7.61 0.15
Thr ACT 84.00 4.77 0.09
Thr ACC 308.00 17.50 0.35

Val GTG 410.00 23.30 0.38
Val GTA 93.00 5.28 0.09
Val GTT 175.00 9.94 0.16
Val GTC 401.00 22.79 0.37

Trp TGG 286.00 16.25 1.00

Tyr TAT 77.00 4.38 0.35
Tyr TAC 143.00 8.13 0.65

End TGA 165.00 9.38 0.67
End TAG 33.00 1.88 0.13
End TAA 49.00 2.78 0.20