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"In silico"


From Wikipedia
If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

In silico
From:"Genomics,Proteomics and Clinical Bacteriology",N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.


Phage Therapy is influenced by:

Phage therapy is influenced by:

Country : the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Temporariness
Mutation rate
Phenotypical delay
Phage cocktail

My point of view

Tuesday, 11 August 2009

To explore one gene of Mycobacterium phage D29. Step 2



*
Go to HAMAP proteomes and write in the upper window : Mycobacterium phage D29 and open.

The page with genes and proteins is showing.


*Open
Q38361 for complete information on Integrase gene of Mycobacterium phage D29.

Read the Function : "
Integrase is necessary for
integration of the phage into the host genome
by site-specific recombination".


Read all points of this page.
For example: the number of amino acids is 333 and it
belongs
to the
'phage' integrase family and it has one active site
for
O-(3'-phospho-DNA)-tyrosine intermediate in position 310.
There is its sequence in Fasta code ( amino acid sequence).



*Go to RefSeq open
NP_046850.1.
and see the function
this gene into the genome:
region name="DNA BRE C" or  "DNA breaking
-rejoining enzymes
,C-terminal catalytic domain.
The DNA breaking-rejoining enzyme superfamily
includes type IB topoisomerases and tyrosine
recombinases
that share the same fold in their catalytic
domain containing six conserved active site...; cl00213"
Open CDD:"140517"


Calculated mol wt= 39869 ( one amino acid is about=
120 and 120 *333= 39960)

Genome Nucleotides=49081
gp33 ( Integrase ) is at 25359-26429 with Locus tag
D29p32.


*
In EBI go to
Integr8, write Mycobacterium phage and open.
Select Mycobacterium phage D29 and open here EMBL.


*In
Q38361 open AF022214_GR and see the contigs and select 33 (Integrase 25359-26429).
Remember: contig is a Group of cloned (copied) pieces of DNA representing overlapping regions of a particular chromosome.


*Open the green line in
Q38361 and see the sequence in amino acids.